Development of Direct Sandwich ELISA test for Detection of Rotavirus in Clinical Specimen

Background and Aims: Rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. Several methods have been used for diagnosis of rotavirus infection. Most of these methods are not easily available in Iran. Methods: In our study attempt was made to develop a method which could replace the commercially imported Kit. Rota virus was purified and nonspecific antibody was prepared. The gamma globulin fraction was purified and conjugated to horse radish peroxidase. Plates were coated with anti-rotavirus gamma globulin and were used for the ELISA method. Results: This method was used for the detection of Rotavirus in clinical specimen. Electron microscopy method was used on the same specimen for observation of Rotavirus particles. Conclusion: It was found that the developed ELISA Kit had a relatively high sensitivity and specificity as compared with Electron Microscopy